Analysis of DAPI Flurescence
| Heat protection |
KG1 |
| Excitation filter |
BG38 + UG1 |
| Dichroic mirror |
TK420 |
| Barrier filter |
GG435 |
Increased sensitivity may be achieved by using a high numerical aperture objective (e.g., Partec 40x1.25 quartz, glycerine).
If needed, excitation light intensity may be decreased by placing a neutral density filter (e.g., NG5) between the UG1 and TK420. This may be necessary when non-linearity of DNA content distributions is observed.
Analysis of Propidium Iodide Flurescence
| Heat protection |
KG1 |
| Excitation filter |
BG38 + EM520 |
| Dichroic mirror |
TK560 |
| Barrier filter |
RG4590 |
The use of a high numeric aperture objective (e.g., Partec 40x1.25 quartz, glycerine) is highly recommended.
If needed, excitation light intensity may be decreased by placing a neutral density filter (e.g., NG5) between the EM520 and TK560. This may be necessary when non-linearity of DNA content distributions is observed.
